Refolding chromatography with immobilized mini-chaperones
نویسندگان
چکیده
منابع مشابه
Refolding chromatography with immobilized mini-chaperones (proteinyrenaturationyfoldingyGroELyhsp60)
Mini-chaperones (e.g., a peptide consisting of residues 191-345 of GroEL) that are immobilized on agarose have very efficient chaperoning activity with several proteins that are otherwise recalcitrant to renaturation by conventional methods. We have used immobilized mini-chaperones both in column chromatography and batchwise to renature an insoluble protein from an inclusion body, to refold app...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1997
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.94.8.3576